E0396
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E0396
Sigma-Aldrich
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid
SigmaUltra, ≥97%
| Synonym: | Chel™-DE, EGTA, Egtazic acid, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid, Glycol ether diamine tetraacetic acid |
| CAS Number: | 67-42-5 |
| Linear Formula: | [-CH2OCH2CH2N(CH2CO2H)2]2 |
| Molecular Weight: | 380.35 |
| Beilstein Registry Number: | 1717370 |
| EC Number: | 200-651-2 |
| MDL number: | MFCD00004291 |
| PubChem Substance ID: | 24894367 |
Description
| Application | A chelating agent useful for the determination of calcium in the presence of magnesium. |
| Legal Information | Chel is a trademark of Ciba-Geigy AG |
Properties
| grade | SigmaUltra |
| assay | ≥97% |
| total impurities | <0.0005% Phosphorus (P) |
| <0.1% Insoluble matter | |
| ign. residue | <0.1% |
| EQP level | Elite |
| mp | 241 °C (dec.)(lit.) |
| solubility | 1 M NaOH: 0.1 M at 20 °C, clear, colorless |
| H2O: increases as pH is increasedsoluble | |
| anion traces | sulfate (SO42-): <0.05% |
| cation traces | Al: <0.0005% |
| Ca: <0.0005% | |
| Cu: <0.0005% | |
| Fe: <0.0005% | |
| K: <0.005% | |
| Mg: <0.0005% | |
| NH4+: <0.05% | |
| Na: <0.05% | |
| Pb: <0.001% | |
| Zn: <0.0005% |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 2 |
| RTECS | AH3760000 |
References
| reference | Brauner, P., Fridlender B., Use of chelating agents as terminators of alkaline phosphatase activity in enzyme-linked immunosorbent assay (ELISA) tests. J. Immunol. Methods 42, 375, (1981) |
| Schmid, R.W., Reilly, C.N. Anal. Chem. 29, 264, (1957) | |
| Riley, W.W., Pfeiffer, D.R., Rapid and extensive release of Ca2+ from energized mitochondria induced by EGTA. J. Biol. Chem. 261, 28, (1986) | |
| Birch-Machin, M.A., Dawson, A.P., Effects of chelating agents on the Ca2+-stimulated ATPase of rat liver plasma membranes. Biochim. Biophys. Acta 855, 277, (1986) | |
| Vidal, H., et al., A test to evaluate the effect of individual components of ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid buffers on enzymatic activity. Anal. Biochem. 193, 135, (1991) | |
| Merck | Merck 13,3560 |
| Beilstein | Beil. 4,IV,2433 |
| Corp MSDS 1, 1609:C / FT-IR 1 (1), 568:C / FT-IR 2 (1), 905:D / NMR-Reference 2 (1), 485:B / RegBook 1 (1), 651:B / Sax 6, 1339 / Sigma FT-IR 1 (2), 552:A / Structure Index 1, 95:C:4 |
